1、Analytical Methods for Brassinosteroid Analysis: Recent Advances and

In general, pollen and immature seeds are particularly rich sources of BRs (1–100 ng/g fresh weight), while concentrations in vegetative tissues are very low (about 0.1 ng/g fresh weight) compared to those of other plant hormones (Bajguz and Tretyn 2003).

2、Analytical Methods for Brassinosteroid Analysis: Recent Advances and

Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants.

3、Protocol for Extraction and Isolation of Brassinosteroids from Plant

Brassinosteroids (BRs) are a class of steroid plant hormones that participate with other plant hormones in the regulation of numerous developmental processes, including root and shoot growth, vascular differentiation, fertility, and seed germination.

4、Comprehensive Overview of the Brassinosteroid Biosynthesis Pathways

Brassinosteroids (BRs) as a class of steroid plant hormones participate in the regulation of numerous developmental processes, including root and shoot growth, vascular differentiation, fertility, flowering, and seed germination, as well as in responding to environmental stresses.

Brassinosteroids: Methods and Protocols

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Brassinosteroid

The application of the brassinosteroid on the crop significantly increases its yield. The use of brassinolide improved the seed weight of the bean crop by almost 50%, and the leaf weight of two distinct kinds of lettuce by approximately 25%.

Phytochemical responses of camelina to brassinolide and boron foliar

To explore the appropriate foliar application of boron (0.5 and 1 %) and 24-Epi-brassinolide (0.5 and 1 μM) and their combinations on camelina (Camelina sativa L.) under irrigation regimes a field experiment was conducted during 2018–2020 years.

Regulation of brassinosteroid biosynthesis and inactivation

In this review, we focus our discussion on a recently-proposed, 8-step predominant BR biosynthetic pathway, several newly-identified transcription factors regulating the expression of key enzymes that catalyze BR biosynthesis, and up-to-date information about the mechanisms that plants use to inactivate unnecessary BRs.

Comprehensive Overview of the Brassinosteroid Biosynthesis Pathways

Comprehensive Overview of the Brassinosteroid Biosynthesis Pathways: Substrates, Products, Inhibitors, and Connections.

Analytical Methods for Brassinosteroid Analysis: Recent Advances and

In general, pollen and immature seeds are particularly rich sources of BRs (1–100 ng/g fresh weight), while concentrations in vegetative tissues are very low (about 0.1 ng/g fresh weight) compared to those of other plant hormones (Bajguz and Tretyn 2003).

Brassinosteroid is a plant growth regulator primarily used to promote plant growth and increase crop yields. In agricultural production, rational use of brassinosteroid can effectively enhance disease resistance, promote root development, improve photosynthesis, and ultimately boost crop yield and quality. the dosage of brassinosteroid must be determined based on specific factors such as crop type, growth stage, and soil conditions to avoid adverse effects on plants.

1. Identify Crop Type

First, clarify the specific crop being cultivated. Different crops respond differently to brassinosteroid, so it is essential to select a product tailored to the target crop. For example, vegetables like tomatoes and cucumbers often show better responses to brassinosteroid, while fruit trees like apples and pears may be less suitable. Choose specialized brassinosteroid products designed for the intended crop to maximize effectiveness.

2. Consider Growth Stage

The timing of brassinosteroid application significantly impacts its efficacy. Applying it during early growth or peak development stages can stimulate growth and yield. using it during late growth or when pests/diseases are present may yield suboptimal results. Understand the crop’s growth stage before application to ensure proper timing.

3. Soil Conditions

Soil type affects the absorption and utilization of brassinosteroid. Loamy or sandy soils typically absorb brassinosteroid more efficiently, allowing for higher doses, while clayey or alkaline soils may require reduced amounts. Additionally, soil moisture, nutrients, and pH influence effectiveness. Conduct soil tests before application to determine optimal dosage.

4. Crop Varieties

Different crop varieties exhibit varying sensitivities to brassinosteroid. Some may be highly sensitive (risk of phytotoxicity), while others tolerate higher doses. Research the specific traits of your crop variety to select appropriate products and application methods.

5. Application Methods

Foliar spraying is the most common method for delivering brassinosteroid, as it quickly enhances yield and quality. environmental factors (e.g., wind, rain) and phytotoxicity risks should be considered. Adjust application methods (e.g., soil drenching, seed treatment) based on specific needs.

6. Precautions

• Avoid Overdosing: Excessive use may inhibit growth or cause phytotoxicity. Follow product guidelines strictly.
• Safety Interval: When mixing with other pesticides, observe recommended waiting periods to prevent reduced efficacy or increased risks.
• Avoid Alkaline Substances: Brassinosteroid degrades in alkaline conditions (e.g., lime solutions), reducing effectiveness.
• Environmental Protection: Minimize residue and contamination by adhering to safe application practices.

Brassinosteroid is a highly effective tool in agriculture, but its use must be tailored to crop type, growth stage, soil conditions, and environmental factors. By prioritizing scientific application and safety, farmers can harness its benefits to improve crop yields and quality, contributing to sustainable agricultural development.

Note: "Mu" (Chinese unit) = approximately 666.67 square meters. Adjust dosages accordingly if converting to metric units (e.g., liters per hectare).